Semrock 滤光片 FF538-FDi02-t3-25x36

538 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Semrock's image-splitting dichroics for super-resolution microscopy deliver industry-leading λ/5 P-V RWE on 3 mm thick substrates for minimal focus shift and optical wavefront aberrations of the reflected imaging beam. These dichroics can be used with much larger diameter imaging beams up to 37 mm while minimizing RWE in custom size versions.

Common Fluorophore Pair to Split: GFP/mOrange

FF538-FDi01 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE

曲线ASC II 参数:measured
类型:Transmission
下载FF538-FDi02-t3-25x36数据: TXT Spectrum data
Semrock网站链接:FF538-FDi02-t3-25x36
型号 尺寸 *未税价格
FF538-FDi02-t3-25x36 25.2 mm x 35.6 mm x 3.0 mm $600.00

* 说明:价格为未包含增值税,关税及运费之价格。

通用规格

参数
size 25.2 mm x 35.6 mm x 3.0 mm
shape rectangular
mounted false
substrate.type low-autofluorescence optical quality glass
scratch-dig 60-40

BrightLineDichroic.Singleband系列参数

参数
ravg-and-passband-range.1 Ravg > 95% 350 – 528.4 nm
edgewl.1 538 nm
tavg-and-passband-range.1 Tavg > 93% 547.7 – 1200 nm
Fluorophore-pair-to-split GFP/mOrange
aoi 45 ± 1.5 degrees
cha 2 degrees
Optical.Damage.Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Steepness Standard
Effective.Index 1.96 Understanding ‘Effective Index of Refraction’ neff
Transverse.Dimensions.(LxW) 25.2 mm x 35.6 mm
Transverse.Tolerance.(mounted) ± 0.1 mm
Filter.Thickness.3.(unmounted) 3.0 mm
Filter.Thickness.Tolerance.3.(unmounted) ± 0.1 mm
Clear.Aperture ≥ 80% (elliptical)
Substrate Thickness (3 mm, unmounted) 3.0 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light
Flatness.RWE Image-splitting
RWE.3 < 0.2λ P-V RWE @ 632.8 nm